Lipid fingerprint image accurately conveys human colon cell pathophysiologic state: A solid candidate as biomarker

por

Trabajo de investigacion · ficha resumen:

Resumen:

Membrane lipids are gaining increasing attention in the clinical biomarker field, as they are associated with different pathologic processes such as cancer or neurodegenerative diseases. Analyzing human colonoscopic sections by matrix assisted laser/desorption ionization (MALDI) mass spectrometry imaging techniques, we identified a defined number of lipid species changing concomitant to the colonocyte differentiation and according to a quite simple mathematical expression.

These species felt into two lipid families tightly associated in signaling: phosphatidylinositols and arachidonic acid-containing lipids. On the other hand, an opposed pattern was observed in lamina propria for AA-containing lipids, coinciding with the physiological distribution of the immunological response cells in this tissue. Importantly, the lipid gradient was accompanied by a gradient in expression of enzymes involved in lipid mobilization. Finally, both lipid and protein gradients were lost in adenomatous polyps. The latter allowed us to assess how different a single lipid species is handled in a pathological context depending on the cell type.

The strict patterns of distribution in lipid species and lipid enzymes described here unveil the existence of fine regulatory mechanisms orchestrating the lipidome according to the physiological state of the cell. In addition, these results provide solid evidence that the cell lipid fingerprint image can be used to predict precisely the physiological and pathological status of a cell, reinforcing its translational impact in clinical research. 

Autores/as (por orden de autoría): Bestard-Escalas J, Garate J, Maimó-Barceló A, Fernández R, Lopez DH, Lage S, Reigada R, Khorrami S, Ginard D, Reyes J, Amengual I, Fernández JA, Barceló- Coblijn G.

Título: Lipid fingerprint image accurately conveys human colon cell pathophysiologic state: A solid candidate as biomarker

Clave: A

Revista: Biochimica et biophysica acta – molecular and cell biology of lipids.

Volumen: 1861

Página inicial: 1942     Página final: 1950

Fecha: Dec 2016

Publicación indexada en JCR: Sí

Base de datos de indexación: JCR Any / Año: 2016

Índice de impacto: 5.547

Posición relativa que ocupa la revista en la categoría: Q1

Categoría: GASTROENTEROLOGY & HEPATOLOGY

Número de citaciones en JCR: 2

Número de citaciones total: 2

Otros indicios de calidad: 

¿Eres médico o profesional sanitario?
¿quieres contactar conmigo, no dudes en hacerlo?

Rellena este formulario y me pondré en contacto contigo lo antes posible.

1 + 13 =