Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5- diaminonaphtalene matrices: application to lipid distribution in human colon
Trabajo de investigacion · ficha resumen:
Resumen:
Imaging mass spectrometry is becoming a reference technique in the field of lipidomics, due to its ability to map the distribution of hundreds of species in a single run, along a tissue section.
The next frontier is now achieving increasing resolution powers to offer cellular (or even sub-cellular) resolution. Thus, the new spectrometers are equipped with sophisticated optical systems to decrease the laser spot to <30 μm. Here, we demonstrate that by using the correct matrix (i.e., a matrix that maximizes ion detection and forms small crystals) and a careful preparation, it is possible to achieve resolutions of ∼5–10 μm, even with spectrometers equipped with non-optimal optics, which produces laser spots of 50 μm or even larger.
As a proof of concept, we present images of distributions of lipids, both in positive and negative ion mode, over human colon endoscopic sections, recorded using 2-mercaptobenzothiazole for positive ion mode and 2,5-diaminonaphtalene for negative ion mode and an LTQ-Orbitrap XL, equipped with a matrix-assisted laser desorption ionization (MALDI) source that produces astigmatic laser spots.
Autores/as (por orden de autoría): : Garate J, Fernández R, Lage S, Bestard-Escalas J, Lopez DH, Reigada R, Khorrami S, Ginard D, Reyes J, Amengual I, Barceló-Coblijn G, Fernández JA.
Título: Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5- diaminonaphtalene matrices: application to lipid distribution in human colon.
Clau / Clave: A
Revista: Analytical and Bioanalytical chemistry
Volumen: 407
Página inicial: 4697 Página final: 4708
Fecha: Jun 2015
Publicación indexada en JCR: Sí
Base de datos de indexación: JCR Any / Año: 2015
Índice de impacto: 3.125
Posición relativa que ocupa la revista en la categoría: Q1
Categoría: GASTROENTEROLOGY & HEPATOLOGY
Número de citaciones en JCR: 9
Número de citaciones total: 9
Otros indicios de calidad: –