Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5- diaminonaphtalene matrices: application to lipid distribution in human colon

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Trabajo de investigacion · ficha resumen:

Resumen:

Imaging mass spectrometry is becoming a reference technique in the field of lipidomics, due to its ability to map the distribution of hundreds of species in a single run, along a tissue section.

The next frontier is now achieving increasing resolution powers to offer cellular (or even sub-cellular) resolution. Thus, the new spectrometers are equipped with sophisticated optical systems to decrease the laser spot to <30 μm. Here, we demonstrate that by using the correct matrix (i.e., a matrix that maximizes ion detection and forms small crystals) and a careful preparation, it is possible to achieve resolutions of ∼5–10 μm, even with spectrometers equipped with non-optimal optics, which produces laser spots of 50 μm or even larger.

As a proof of concept, we present images of distributions of lipids, both in positive and negative ion mode, over human colon endoscopic sections, recorded using 2-mercaptobenzothiazole for positive ion mode and 2,5-diaminonaphtalene for negative ion mode and an LTQ-Orbitrap XL, equipped with a matrix-assisted laser desorption ionization (MALDI) source that produces astigmatic laser spots.

Autores/as (por orden de autoría): : Garate J, Fernández R, Lage S, Bestard-Escalas J, Lopez DH, Reigada R, Khorrami S, Ginard D, Reyes J, Amengual I, Barceló-Coblijn G, Fernández JA.

Título: Imaging mass spectrometry increased resolution using 2-mercaptobenzothiazole and 2,5- diaminonaphtalene matrices: application to lipid distribution in human colon.

Clau / Clave: A

Revista: Analytical and Bioanalytical chemistry

Volumen: 407

Página inicial: 4697   Página final: 4708

Fecha: Jun 2015

Publicación indexada en JCR: Sí

Base de datos de indexación: JCR Any / Año: 2015

Índice de impacto: 3.125

Posición relativa que ocupa la revista en la categoría: Q1

Categoría: GASTROENTEROLOGY & HEPATOLOGY

Número de citaciones en JCR: 9

Número de citaciones total: 9

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